{
  "_id": "6a1f1fbab401979e734201ef",
  "Package": "ezmmek",
  "Title": "Easy Michaelis-Menten Enzyme Kinetics",
  "Version": "0.2.4",
  "Authors@R": "c(person(given = \"Christopher\", family = \"Cook\", email = \"ccook62@vols.utk.edu\",\nrole = c(\"aut\", \"cre\")),\nperson(given = \"Andrew\", family = \"Steen\", email = \"asteen1@utk.edu\",\nrole = c(\"aut\")))",
  "Description": "Serves as a platform for published fluorometric enzyme\nassay protocols. 'ezmmek' calibrates, calculates, and plots\nenzyme activities as they relate to the transformation of\nsynthetic substrates. At present, 'ezmmek' implements two\ncommon protocols found in the literature, and is modular to\naccommodate additional protocols. Here, these protocols are\nreferred to as the In-Sample Calibration (Hoppe, 1983;\n<doi:10.3354/meps011299>) and In-Buffer Calibration (German et\nal., 2011; <doi:10.1016/j.soilbio.2011.03.017>). protocols. By\ncontaining multiple protocols, 'ezmmek' aims to stimulate\ndiscussion about how to best optimize fluorometric enzyme\nassays. A standardized approach would make studies more\ncomparable and reproducible.",
  "License": "AGPL-3",
  "Encoding": "UTF-8",
  "Language": "en-US",
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  "VignetteBuilder": "knitr",
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  "Packaged": {
    "Date": "2026-05-16 08:03:12 UTC",
    "User": "root"
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  "Author": "Christopher Cook [aut, cre], Andrew Steen [aut]",
  "Maintainer": "Christopher Cook <ccook62@vols.utk.edu>",
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  "Repository": "https://ccook.r-universe.dev",
  "Date/Publication": "2020-08-28 06:10:03 UTC",
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    {
      "package": "knitr",
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    },
    {
      "package": "rmarkdown",
      "role": "Suggests"
    },
    {
      "package": "testthat",
      "role": "Suggests"
    }
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  "_selfowned": true,
  "_usedby": 0,
  "_updates": [],
  "_tags": [],
  "_stars": 0,
  "_contributors": [
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      "uuid": 56552289
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  "_userbio": {
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    "type": "user",
    "name": "Christopher L. Cook"
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    "source": "https://cranlogs.r-pkg.org/downloads/total/last-month/ezmmek"
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  "_rbuild": "4.6.0",
  "_assets": [
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    "extra/citation.json",
    "extra/citation.txt",
    "extra/contents.json",
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  "_realowner": "ccook",
  "_cranurl": false,
  "_releases": [
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      "date": "2019-12-20"
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      "date": "2020-08-28"
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    "new_ezmmek_act_calibrate",
    "new_ezmmek_act_group",
    "new_ezmmek_sat_fit",
    "new_ezmmek_std_group"
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    {
      "page": "ezmmek_calc_mm_fit",
      "title": "ezmmek_calc_mm_fit",
      "topics": [
        "ezmmek_calc_mm_fit"
      ]
    },
    {
      "page": "ezmmek_calc_std_lm_buffer",
      "title": "ezmmek_calc_std_lm_homo",
      "topics": [
        "ezmmek_calc_std_lm_buffer"
      ]
    },
    {
      "page": "ezmmek_calc_std_lm_homo",
      "title": "ezmmek_calc_std_lm_homo",
      "topics": [
        "ezmmek_calc_std_lm_homo"
      ]
    },
    {
      "page": "ezmmek_calc_std_lm_homo_buffer",
      "title": "ezmmek_calc_std_lm_homo_buffer",
      "topics": [
        "ezmmek_calc_std_lm_homo_buffer"
      ]
    },
    {
      "page": "ezmmek_calibrate_activities",
      "title": "ezmmek_calibrate_activities",
      "topics": [
        "ezmmek_calibrate_activities"
      ]
    },
    {
      "page": "ezmmek_std_lm",
      "title": "ezmmek_std_lm",
      "topics": [
        "ezmmek_std_lm"
      ]
    },
    {
      "page": "new_ezmmek_act_calibrate",
      "title": "new_ezmmek_act_calibrate",
      "topics": [
        "new_ezmmek_act_calibrate"
      ]
    },
    {
      "page": "new_ezmmek_act_group",
      "title": "new_ezmmek_act_group",
      "topics": [
        "new_ezmmek_act_group"
      ]
    },
    {
      "page": "new_ezmmek_sat_fit",
      "title": "new_ezmmek_sat_fit",
      "topics": [
        "new_ezmmek_sat_fit"
      ]
    },
    {
      "page": "new_ezmmek_std_group",
      "title": "new_ezmmek_std_group",
      "topics": [
        "new_ezmmek_std_group"
      ]
    },
    {
      "page": "plot.new_ezmmek_act_group",
      "title": "plot_new_ezmmek_act_group",
      "topics": [
        "plot.new_ezmmek_act_group"
      ]
    },
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      "page": "plot.new_ezmmek_calibrate",
      "title": "plot_new_ezmmek_calibrate",
      "topics": [
        "plot.new_ezmmek_calibrate"
      ]
    },
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      "title": "plot_new_ezmmek_sat_fit",
      "topics": [
        "plot.new_ezmmek_sat_fit"
      ]
    },
    {
      "page": "plot.new_ezmmek_std_group",
      "title": "plot_new_ezmmek_std_group",
      "topics": [
        "plot.new_ezmmek_std_group"
      ]
    }
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    "data.table",
    "dplyr",
    "farver",
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    "glue",
    "gtable",
    "isoband",
    "labeling",
    "lifecycle",
    "magrittr",
    "nls2",
    "pillar",
    "pkgconfig",
    "proto",
    "purrr",
    "R6",
    "RColorBrewer",
    "rlang",
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    "stringi",
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    "tidyr",
    "tidyselect",
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      "source": "ezmmek_vignette.Rmd",
      "filename": "ezmmek_vignette.html",
      "title": "ezmmek tutorial",
      "engine": "knitr::rmarkdown",
      "headings": [
        "1 Introduction",
        "1.1 In-Buffer Calibration protocol:",
        "1.2 In-Sample Calibration protocol:",
        "2 Installation",
        "2.1 CRAN",
        "2.2 GitHub",
        "2.2.1 Install and load devtools",
        "2.2.2 Install ezmmek",
        "2.2.3 Required packages",
        "3 How to use",
        "3.1 Load ezmmek",
        "3.2 Example datasets",
        "3.2.1 Standard curve data",
        "3.2.2 Raw fluorescence data, IBC protocol",
        "3.2.3 Raw fluorescence data, ISC protocol",
        "3.3 Visible functions",
        "3.3.1 Create data.frame object of class new_ezmmek_sat_fit",
        "3.3.2 Create data.frame object of class new_ezmmek_calibrate",
        "3.3.3 Create data.frame object of class new_ezmmek_act_group",
        "3.3.4 Create data.frame object of class new_ezmmek_std_group",
        "4 Example analyses",
        "4.1 new_ezmmek_sat_fit",
        "4.1.1 new_ezmmek_sat_fit, IBC",
        "4.1.2 new_ezmmek_sat_fit, ISC",
        "4.2 new_ezmmek_act_calibrate",
        "4.2.1 new_ezmmek_act_calibrate, IBC",
        "4.2.2 new_ezmmek_act_calibrate, ISC",
        "4.3 new_ezmmek_act_group",
        "4.3.1 new_ezmmek_act_group, IBC",
        "4.3.2 new_ezmmek_act_group, ISC",
        "4.4 new_ezmmek_std_group",
        "4.4.1 new_ezmmek_std_group, IBC",
        "4.4.2 new_ezmmek_std_group, ISC",
        "5 Methods for new_ezmmek objects",
        "5.1 plot",
        "5.1.1 new_ezmmek_sat_fit, IBC and ISC",
        "5.1.2 new_ezmmek_calibrate, IBC and ISC",
        "5.1.3 new_ezmmek_act_group, IBC and ISC",
        "5.1.4 new_ezmmek_std_group, IBC and ISC",
        "6 References",
        "7 Authors",
        "8 License"
      ],
      "created": "2020-08-28 06:10:03",
      "modified": "2020-08-28 06:10:03",
      "commits": 1
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